Regulatory
MS toehold
Part:BBa_K5106003:Design
Designed by: Ivar Gruppen Group: iGEM24_WageningenUR (2024-09-29)
Toehold switch C for detection of hsa-miR-484.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The toehold switch should form a hairpin structure, where the base of the bottom stem structure. It should the basic design of the second generation toehold switch of Pardee et al. 2016.1 The minimum free energy of the complex of toehold switch annealed to the miRNA should also be lower compared to the MFE of the toehold switch and miRNA alone in the solution.
Source
Designed by miRADAR